Mechano-mimetic cell culture: textured rigidity​

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Micropatterned polyacrylamide gel with fluorescent carboxylate-modified microspheres uniformly dispersed inside.

TRACTION FORCE MICROSCOPY

BENEFITS

• Robust & reliable

• Ready & easy to use

• No biological risk (synthetic matrix)

• Glass bottom: ideal for microscopy

• Stiffness and coating are decoupled

STORAGE

• Temperature: +4°C

• Shelf life: 3 months

SHAPES*

• Subcellular to cellular scale (1 - 100 μm): geometrics patterns (dots, triangles, stripes)

• Tissue scale (Pa/mm to kPa/μm): steps, gradients

*Custom patterns upon request

BEADS

• Size: 0.2 µm

• Fluorophore: dark red

• Wavelength: Exit. / Emiss. ; 660 / 680 nm

• Cell seeding surface: 6.15 cm2

• Stiffness and coating are decoupled

*Specific beads upon request

CHOOSE YOUR STIFFNESS

Uniform rigidity:

• 8 kPa

• 10 kPa

• 12 kPa

*Specific stiffness upon request

Rigidity step:​

• 1 kPa | 1.5 kPa | 2 kPa | 3.5 kPa | 4 kPa

• 5 kPa | 6.5 kPa | 7.5 kPa | 8 kPa | 9.5 kPa

CHOOSE YOUR COATING

Culture dishes and plates are pre-coated with ECM protein or synthetic amino acid.

Culture dedicated surface chemistry:

• Vitronectin (human; recombinant truncated)

• Fibronectin (human; plasma)

• Collagen I (rat; tail)

• Laminin (mouse; EHC sarcoma)

• Poly-Ornithine | Laminin

• Poly-Ornithine

UNIQUE FORMAT

• PD35