µpattern MecaTract

 

FOR TRACTION FORCE MICROSCOPY AT THE MICROMETER SCALE

by Cell&Soft

µpattern MecaTract.png

Mechano-mimetic cell culture: textured rigidity

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DESCRIPTION

Micropatterned polyacrylamide gel with fluorescent carboxylate-modified microspheres uniformly dispersed inside.

APPLICATIONS

TRACTION FORCE MICROSCOPY

CHARACTERISTICS

BENEFITS

  • Robust & reliable

  • Ready & easy to use

  • No biological risk (synthetic matrix)

  • Glass bottom: ideal for microscopy

  • Stiffness and coating are decoupled

STORAGE

  • Temperature: +4°C

  • Shelf life: 3 months

SHAPES*

  • Subcellular to cellular scale (1 - 100 μm): geometrics patterns (dots, triangles, stripes)

  • Tissue scale (Pa/mm to kPa/μm): steps, gradients

*Custom patterns upon request

BEADS

  • Size: 0.2 µm

  • Fluorophore: dark red

  • Wavelength: Exit. / Emiss. ; 660 / 680 nm

  • Cell seeding surface: 6.15 cm2

  • Stiffness and coating are decoupled

*Specific beads upon request

CUSTOMIZATION

CHOOSE YOUR STIFFNESS

Uniform rigidity:

  • 8 kPa

  • 10 kPa

  • 12 kPa

*Specific stiffness upon request

Rigidity step:

  • 1 kPa | 1.5 kPa | 2 kPa | 3.5 kPa | 4 kPa

  • 5 kPa | 6.5 kPa | 7.5 kPa | 8 kPa | 9.5 kPa

CHOOSE YOUR COATING

Culture dishes and plates are pre-coated with ECM protein or synthetic amino acid.

Culture dedicated surface chemistry:

  • Vitronectin (human; recombinant truncated)

  • Fibronectin (human; plasma)

  • Collagen I (rat; tail)

  • Laminin (mouse; EHC sarcoma)

  • Poly-Ornithine | Laminin

  • Poly-Ornithine

UNIQUE FORMAT

  • PD35

PRODUCT SHEET

Download product sheet here.

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