Uniform rigidity : The MecachipsTM
Cell&Soft provides uniformly soft or rigidity-textured cell culture plates, with an adhesive coating.
The culture plates offer mechanical features as close as possible to those found in vivo in animal or human tissues.
CHOOSE YOUR ORGAN OF INTEREST
Choose between 11 rigidities available, according to your
organ of interest from brain to cartilage :
CHOOSE YOUR COATING
The surface density of the coating is
independent of the rigidity :
Vitronectin (human truncated recombinant)
Laminin (mouse EHS sarcoma)
Collagen I (rat tail)
Fibronectin (human plasma)
CHOOSE YOUR FORMAT
Available in standard format*
Compatible with standard analysis
Scalable to screening workflow
*p384 upon request
Textured rigidity : The µpattern MecachipsTM
Zoom on the local stiffness of a human pituitary gland (hypohysis) tissue,
measured by force indentation atomic force microscopy. Adapted from 2 :
M. Moussus, C. der Loughian, D. Fuard, M. Courcon, D. Gulino-Debrac, H. Delanoe-Ayari, and A. Nicolas . Intracellular stresses in patterned cell assemblies. Soft Matter, 10 :2414–2423, 2014.
M. Moussus, C. der Loughian, D. Fuard, M. Courçon, D. Gulino Debrac, H. Delanoë-Ayari, and A. Nicolas. Reply to the ’comment on "intracellular stresses in patterned cell assemblies"’ by D. Tambe et al., Soft Matter, 2014, 10, 7681. Soft Matter, 10 :7683–7684, 2014.
N. Bouchonville, M. Meyer, C. Gaude, E. Gay, D. Ratel, and A. Nicolas. AFM mapping of the elastic properties of brain tissue reveals kPa/µm gradients of rigidity. Soft Matter, 12 :6232–6239, 2016.
A. Nicolas. Cell adhesion mechanosensitivity, an active biological process. Comment on ”cellular mechanosensing of the biophysical microenvironment : A review of the mathematical models of biophysical regulation of cell responses” by Bo Cheng et al. Phys. Life Rev., 22-23 :123–126, 2017.
N. Bouchonville and A. Nicolas . Quantification of the elastic properties of soft and sticky materials using AFM. In N. C. Santos and F. A. Carvalho, editors, Meth. Mol. Biol., Atomic Force Microscopy : Methods and Protocols, 281–290, 2019.
C. Tomba, C. Migdal, D. Fuard, C. Villard, and A. Nicolas. Glial cell mechanosensitivity is reversed by adhesion cues. BioRxiv, 865303, 2019.
S. Palva, P. Joanne, C. Migdal, E. Lopez-Soler, Y. Hovhannisyan, A. Nicolas, and O. Agbulut . Polyacrylamide hydrogels with rigidity-independent surface chemistry show limited long-term maintenance of pluripotency of human induced pluripotent stem cells on soft substrate. ACS Biomat. Sci. Eng., 6 :340-351, 2020.