Cell&Soft provides uniformly soft or rigidity textured cell culture plates, with an
The culture plates offer mechanical features as close as possible to those found in vivo in animal or human tissues.
CHOOSE YOUR RIGIDITY
Choose between 11 rigidities available, according to your organ of interest from brain to cartilage.
CHOOSE YOUR FORMAT
CHOOSE YOUR COATING
Choose between 6 coatings available. The surface density of the coating is independent of the rigidity.
Available in standard format*
Compatible with standard analysis
Scalable to screening workflow
PROTEINOGENIC AMINO ACID
Collagen I (rat tail)
Vitronectin (human truncated recombinant)
Fibronectin (human plasma)
Laminin (mouse EHS sarcoma)
NON-PROTEINOGENIC AMINO ACID
*p384 upon request
Zoom on the local stiffness of a human pituitary gland (hypohysis) tissue, measured by force indentation atomic force microscopy. Adapted from 2.
M. Moussus, C. der Loughian, D. Fuard, M. Courcon, D. Gulino-Debrac, H. Delanoe-Ayari, and A. Nicolas . Intracellular stresses in patterned cell assemblies. Soft Matter, 10 :2414–2423, 2014.
M. Moussus, C. der Loughian, D. Fuard, M. Courçon, D. Gulino Debrac, H. Delanoë-Ayari, and A. Nicolas. Reply to the ’comment on "intracellular stresses in patterned cell assemblies"’ by D. Tambe et al., Soft Matter, 2014, 10, 7681. Soft Matter, 10 :7683–7684, 2014.
N. Bouchonville, M. Meyer, C. Gaude, E. Gay, D. Ratel, and A. Nicolas. AFM mapping of the elastic properties of brain tissue reveals kPa/µm gradients of rigidity. Soft Matter, 12 :6232–6239, 2016.
A. Nicolas. Cell adhesion mechanosensitivity, an active biological process. Comment on ”cellular mechanosensing of the biophysical microenvironment : A review of the mathematical models of biophysical regulation of cell responses” by Bo Cheng et al. Phys. Life Rev., 22-23 :123–126, 2017.
N. Bouchonville and A. Nicolas . Quantification of the elastic properties of soft and sticky materials using AFM. In N. C. Santos and F. A. Carvalho, editors, Meth. Mol. Biol., Atomic Force Microscopy : Methods and Protocols, 281–290, 2019.
C. Tomba, C. Migdal, D. Fuard, C. Villard, and A. Nicolas. Glial cell mechanosensitivity is reversed by adhesion cues. BioRxiv, 865303, 2019.
S. Palva, P. Joanne, C. Migdal, E. Lopez-Soler, Y. Hovhannisyan, A. Nicolas, and O. Agbulut . Polyacrylamide hydrogels with rigidity-independent surface chemistry show limited long-term maintenance of pluripotency of human induced pluripotent stem cells on soft substrate. ACS Biomat. Sci. Eng., 6 :340-351, 2020.