

Mechano-mimetic cell culture: uniform rigidity

Hypophysis
Zoom on the local stiffness of a human pituitary gland (hypophysis) tissue, measured by force indentation atomic force microscopy. Adapted from 2.
DESCRIPTION
Cell&Soft culture plates offer mechanical features as close as possible to those found in vivo in animal or human tissues. Mecachips® soft and flat matrices are new and physiological solutions for in vitro cell culture. They mimic the soft mechanical features of all human or animal tissues, thus preserving the cells in vivo characteristics.
APPLICATIONS
AREAS
Basic research, stem cells, oncology, neurology, cardiology, compatible with HCS/HTS platforms.
TOOLS
Cell biology, molecular biology, biochemistry.
CHARACTERISTICS
BENEFITS
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Robust & reliable
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Ready & easy to use
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Scalable to screening workflow (no topography)
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No biological risk (synthetic matrix)
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Glass bottom: ideal for microscopy
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Stiffness and coating are decoupled
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Compatible with standard analysis
STORAGE
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Temperature: +4°C
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Shelf life: 3 months
CUSTOMIZATION
CHOOSE YOUR STIFFNESS
Stiffness according to the organ of interest:
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1 kPa (breast)
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3 kPa (liver)
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3,5 kPa (pancreas)
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4 kPa (lung)
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5 kPa (artery | skin)
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6 kPa (brain)
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8 kPa (spleen)
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9 kPa (kidney)
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10 kPa (heart)
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>25 kPa (cartilage)
CHOOSE YOUR COATING
Culture dishes and plates are pre-coated with ECM protein or synthetic amino acid.
Culture dedicated surface chemistry:
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Vitronectin (human; recombinant truncated)
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Fibronectin (human; plasma)
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Collagen I (rat; tail)
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Laminin (mouse; EHC sarcoma)
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Poly-Ornithine | Laminin
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Poly-Ornithine
CHOOSE YOUR FORMAT
Standards formats*
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PD35
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P6
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P12
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P24
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P48
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P96
*P384 upon request
PRODUCT SHEET
In vivo, cells lay in soft tissues with distinct physical properties. Rigidity plays a major role in a myriad of cellular mechanisms, such as carcinogenesis and metastasis formation, as well as stem cells differentiation and drug effectiveness. To be representative, in vitro cell devices should selectively provide culture conditions as close as possible to the mechanical microenvironment of targeted tissues.
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Soft and rigidity micropatterned culture plates

MecaTract
Polyacrylamide gel with fluorescent beads dispersed inside for Traction Force Microscopy.
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