by Cell&Soft

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TRACTION FORCE MICROSCOPY

Polyacrylamide gel with fluorescent carboxylate-modified microspheres uniformly dispersed inside.

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Product information

Description

Cell-contractile forces generated by the actomyosin cytoskeleton and transmitted to the extracellular matrix (ECM) drive cell adhesion, spreading and migration. These forces are known to be critical during embryo morphogenesis, wound healing, immune response as well as pathological processes such as cancer metastasis. Traction force microscopy (TFM) is a recognized experimental technique that measures the surface forces, also termed as tractions, that cells exert on a given substrate. It relies on the computational analysis of the direction and magnitude of elastic substrate deformations to reconstruct cell-generated traction forces. These deformations can be tracked and quantified by recording the displacement of fluorescent beads already embedded in the substrate, as a result of the mechanical stress induced by an adherent cell.

Beads

SIZE

0.2 µm

FLUOROPHORE

Dark red

WAVELENGTH 

Exit. / Emiss.: 660 / 680 nm

CELL SEEDING SURFACE

6.15 cm2

*Specific beads on request

Applications

TRACTION FORCE MICROSCOPY

Characteristics

BENEFITS

  • Robust & reliable

  • Ready & easy to use

  • No biological risk (synthetic matrix)

  • Glass bottom: ideal for microscopy

  • Stiffness and coating are decoupled

STORAGE

  • Temperature: +4°C

  • Shelf life: 3 months

Customization

Choose your stiffness

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1

STANDARD STIFFNESSES*

*Specific stiffness on request

  • 8 kPa

  • 10 kPa

  • 12 kPa

Choose your coating

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2

CULTURE DEDICATED SURFACE CHEMISTRY

Culture dishes and plates are pre-coated with ECM protein or synthetic amino acid:

  • Fibronectin (human, recombinant truncated)

  • Collagen I (rat, tail)

  • Laminin (mouse, EHS sarcoma)

  • Poly-Ornithine / Laminin

  • Poly-Ornithine

Choose your format

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3

ONE FORMAT

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PD35

Technical sheet

Download technical sheet here.