MecaTract

 

FOR TRACTION FORCE MICROSCOPY

by Cell&Soft

MecaTract.png

Mechano-mimetic cell culture.
Traction Force Microscopy.

Cell-contractile forces generated by the actomyosin cytoskeleton and transmitted to the extracellular matrix (ECM) drive cell adhesion, spreading, and migration. These forces are known to be critical during embryo morphogenesis, wound healing, immune response as well as pathological processes, such as cancer metastasis. Traction force microscopy (TFM) is a recognized experimental technique that measures the surface forces, also termed as tractions, that cells exert on a substrate. It relies on the computational analysis of the direction and the magnitude of elastic substrate deformations to reconstruct cell-generated traction forces. These deformations can be tracked and quantified by recording the displacement of fluorescent beads embedded in the substrate, as a result of the mechanical stress induced by an adherent cell.

DESCRIPTION

Polyacrylamide gel with fluorescent carboxylate-modified microspheres uniformly dispersed inside.

APPLICATIONS

Traction Force Microscopy.

CHARACTERISTICS

BENEFITS | Relevant, robust, reliable, plug & play, compliant, disruptive, no biological risk (synthetic matrix), glass bottom: ideal for microscopy, stiffness and coating are decoupled.

STORAGE | Temperature: +4°C. Shelf life: 3 months.

BEADS* | Size: 0.2 µm. Fluorophore: dark red. Wavelength: Exit. / Emiss. ; 660 / 680 nm. Cell seeding surface: 6.15 cm².

*Specific beads on request

CUSTOMIZATION

1

CHOOSE YOUR STIFFNESS

Standards stiffnesses:

8 kPa

10 kPa

12 kPa

*Specifics stiffnesses on request

Culture dishes and plates are pre-coated with ECM protein or synthetic amino acid. Culture dedicated surface chemistry:

2

CHOOSE YOUR COATING

Vitronectin

Human; recombinant truncated

Human; plasma

Fibronectin

Collagen I

Rat; tail

Laminin

Mouse; EHC sarcoma

Poly-Ornithine | Laminin

Poly-Ornithine

Uniform rigidity:

3

UNIQUE

PD35

FORMAT

BP35.png

Rigidity step:

µpattern MecaTract_Rigidity step.png
 

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