

Mechano-mimetic cell culture: Traction Force Microscopy
Cell-contractile forces generated by the actomyosin cytoskeleton and transmitted to the extracellular matrix (ECM) drive cell adhesion, spreading, and migration. These forces are known to be critical during embryo morphogenesis, wound healing, immune response as well as pathological processes, such as cancer metastasis. Traction force microscopy (TFM) is a recognized experimental technique that measures the surface forces, also termed as tractions, that cells exert on a substrate. It relies on the computational analysis of the direction and the magnitude of elastic substrate deformations to reconstruct cell-generated traction forces. These deformations can be tracked and quantified by recording the displacement of fluorescent beads embedded in the substrate, as a result of the mechanical stress induced by an adherent cell.
DESCRIPTION
Polyacrylamide gel with fluorescent carboxylate-modified microspheres uniformly dispersed inside.
APPLICATIONS
TRACTION FORCE MICROSCOPY
CHARACTERISTICS
BENEFITS
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Robust & reliable
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Ready & easy to use
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No biological risk (synthetic matrix)
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Glass bottom: ideal for microscopy
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Stiffness and coating are decoupled
STORAGE
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Temperature: +4°C
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Shelf life: 3 months
BEADS
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Size: 0.2 µm
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Fluorophore: dark red
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Wavelength: Exit. / Emiss. ; 660 / 680 nm
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Cell seeding surface: 6.15 cm2
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Stiffness and coating are decoupled
*Specific beads upon request
CUSTOMIZATION
CHOOSE YOUR STIFFNESS
Standards stiffnesses:
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8 kPa
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10 kPa
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12 kPa
*Specific stiffnesses upon request
CHOOSE YOUR COATING
Culture dishes and plates are pre-coated with ECM protein or synthetic amino acid.
Culture dedicated surface chemistry:
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Vitronectin (human; recombinant truncated)
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Fibronectin (human; plasma)
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Collagen I (rat; tail)
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Laminin (mouse; EHC sarcoma)
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Poly-Ornithine | Laminin
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Poly-Ornithine
UNIQUE FORMAT
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PD35
PRODUCT SHEET
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