Cell&Soft soft bottoms are made of polyacrylamide, bound to a 150µm thin glass coverslip.
Consistent mechanical properties
The hydrogel is photo-polymerized, to ensure uniform rigidity and reproducibility.
Meeting high quality standards
Cell&Soft culture plates are manufactured in dust free, ISO7, clean room environment.
Consistent surface chemistry
The culture plates are pre-coated with adhesive molecules. Cell&Soft guarantees that the surface density of the coating is independent of the rigidity.
Lot number traceability
A lot number is attributed to every product to ensure traceability. Series can be booked.
SURFACE COATING OF THE POLYACRYLAMIDE HYDROGEL
The human truncated recombinant vitronectin (VTN-N) is covalently grafted to the surface and does not penetrate into the hydrogel. Horizontal bar: 50µm. Vertical bar: 10µm.
The surface density of the VTN-N is independent of the rigidity of the hydrogel.
EXEMPLE OF APPLICATION : COMPATIBLE WITH HOLOGRAPHIC MICROSCOPY SYSTEMS
Courtesy of System C Bioprocess, France and PHI AB (Phase Holography Imaging), Sweden
Cells: A549 (adenocarcinomic human alveolar basal epithelial cells)
Plate: Lung Mecachips™ - Collagen
Labeling: Label free
Microscopy: Holomonitor™ M4 (PHI AB (Sweden), distributor: System C (France)) https://www.system-c-bioprocess.com/produit/holomonitor-m4/
Equipment: motarized stage, low power red laser, x20 magnification objective
Time-Lapse imaging: App Suite – 1 image every 5 minutes – 24 h