Soft culture plates for pleased cells
READY TO USE
EASY TO USE
In vitro cell-based assays are recognized as important contributors to persistent failures to translate promising drug candidates into clinical success, due, in part, to their limited predictability and reproducibility of the in vivo cell models.
In biological conditions, cells organize in tissues with distinct physical properties where they evolve on soft and textured matrices2. Matrix rigidities impact almost all aspect of cell behavior1, including transcriptional regulation3, protein synthesis4, endocytosis5 and even glycolysis necessary for fast metabolism6. They play a major role in carcinogenesis and metastasis formation7, in stem cells differentiation8 and
in drug effectiveness9. Therefore, cell culture devices should not only allow cell survival and growth but must offer an environment as close as possible to the natural microenvironment of targeted tissues to be representative of the in vivo conditions.
Mean elastic Young’s moduli of human organs
Up to date, cell-based assays are still performed in plastic dishes, whose rigidity is up to one billion times stiffer than the in vivo cell environment! The failures of standard culture dishes to maintain the phenotype of many primary cells, or by appearing as wrong predictors support the evidence than these standard tools have now reached their limits. Then, now,
let’s reinvent cell culture…
…with using soft, mechano-mimetic culture plates!
Discher DE et al. Tissue cells feel and respond to the stiffness of their substrate. Science. 2005 310:1139.
Bouchonville N et al. AFM mapping of the elastic properties of brain tissue reveals kPa/μm gradients of rigidity. Soft Matter. 2016 12:6232.
Mammoto A et al. Mechanosensitive mechanisms in transcriptional regulation. J Cell Sci. 2012 125:3061.
Tilghman RW et al. Matrix rigidity regulates cancer cell growth by modulating cellular metabolism and protein synthesis.PLoS One. 2012 7(5):e37231.
Missirlis D. The effect of substrate elasticity and actomyosin contractility on different forms of endocytosis. PLoS One. 2014 9(5):e96548.
Park JS et al. Mechanical regulation of glycolysis via cytoskeleton architecture. Nature. 2020 578 :621-626
Mouw JK et al. Tissue mechanics modulate microRNA-dependent PTEN expression to regulate malignant progression. Nat Med. 2014 20:360.
Engler AJ et al. Matrix elasticity directs stem cell lineage specification. Cell. 2006 126(4):677-89.
Zustiak SP. The Role of Matrix Compliance on Cell Responses to Drugs and Toxins: Towards Predictive Drug Screening Platforms. Macromol Biosci. 2015 15:589-99.
Cell&Soft soft bottoms are made of polyacrylamide, bound to a 150µm thin glass coverslip.
Consistent mechanical properties
The hydrogel is photo-polymerized, to ensure uniform rigidity and reproducibility.
Meeting high quality standards
Cell&Soft culture plates are manufactured in dust free, ISO7, clean room environment.
Consistent surface chemistry
The culture plates are pre-coated with adhesive molecules. Cell&Soft guarantees that the surface density of the coating is independent of the rigidity.
Lot number traceability
A lot number is attributed to every product to ensure traceability. Series can be booked.
SURFACE COATING OF THE POLYACRYLAMIDE HYDROGEL
The human truncated recombinant vitronectin (VTN-N) is covalently grafted to the surface and does not penetrate into the hydrogel. Horizontal bar: 50µm. Vertical bar: 10µm.
The surface density of the VTN-N is independent of the rigidity of the hydrogel.
Cell&Soft provides uniformly soft or rigidity-textured cell culture plates, with an adhesive coating.
The culture plates offer mechanical features as close as possible to those found in vivo in animal or human tissues.
CHOOSE YOUR ORGAN OF INTEREST
Choose between 10 rigidities available, according to your
organ of interest from brain to cartilage :
CHOOSE YOUR COATING
The surface density of the coating is
independent of the rigidity.
Fibronectin (human plasma)
Laminin (mouse EHS sarcoma)
Collagen I (rat tail)
Vitronectin (human truncated recombinant)
CHOOSE YOUR FORMAT
Available in standard format
Compatible with standard analysis
Scalable to screening workflow
Zoom on the local stiffness of a human pituitary gland (hypohysis) tissue,
measured by force indentation atomic force microscopy. Adapted from 2 :
Cell&Soft is a young French Biotech, located in the Auvergne Rhone Alpes region, in Grenoble City. Born from a joint project between LTM Lab (CNRS) and CEA, Cell&Soft is specialized in the development, production and sale of soft and biomechanical substrates for in vitro cell culture. Cell&Soft offers solutions to enhance the physiology and the relevance of cell culture and cell-based assays.