Discher DE et al. Tissue cells feel and respond to the stiffness of their substrate. Science. 2005 310:1139.
Bouchonville N et al. AFM mapping of the elastic properties of brain tissue reveals kPa/μm gradients of rigidity. Soft Matter. 2016 12:6232.
Mammoto A et al. Mechanosensitive mechanisms in transcriptional regulation. J Cell Sci. 2012 125:3061.
Tilghman RW et al. Matrix rigidity regulates cancer cell growth by modulating cellular metabolism and protein synthesis.PLoS One. 2012 7(5):e37231.
Missirlis D. The effect of substrate elasticity and actomyosin contractility on different forms of endocytosis. PLoS One. 2014 9(5):e96548.
Park JS et al. Mechanical regulation of glycolysis via cytoskeleton architecture. Nature. 2020 578 :621-626
Mouw JK et al. Tissue mechanics modulate microRNA-dependent PTEN expression to regulate malignant progression. Nat Med. 2014 20:360.
Engler AJ et al. Matrix elasticity directs stem cell lineage specification. Cell. 2006 126(4):677-89.
Zustiak SP. The Role of Matrix Compliance on Cell Responses to Drugs and Toxins: Towards Predictive Drug Screening Platforms. Macromol Biosci. 2015 15:589-99.
Soft culture plates for pleased cells
READY TO USE
EASY TO USE
In vitro cell-based assays are recognized as important contributors to persistent failures to translate promising drug candidates into clinical success, due, in part, to their limited predictability and reproducibility of the in vivo cell models.
In biological conditions, cells organize in tissues with distinct physical properties where they evolve on soft and textured matrices2. Matrix rigidities impact almost all aspect of cell behavior1, including transcriptional regulation3, protein synthesis4, endocytosis5 and even glycolysis necessary for fast metabolism6. They play a major role in carcinogenesis and metastasis formation7, in stem cells differentiation8 and
in drug effectiveness9. Therefore, cell culture devices should not only allow cell survival and growth but must offer an environment as close as possible to the natural microenvironment of targeted tissues to be representative of the in vivo conditions.
Mean elastic Young’s moduli of human organs
Up to date, cell-based assays are still performed in plastic dishes, whose rigidity is up to one billion times stiffer than the in vivo cell environment! The failures of standard culture dishes to maintain the phenotype of many primary cells, or by appearing as wrong predictors support the evidence than these standard tools have now reached their limits. Then, now,
let’s reinvent cell culture…
…with using soft, mechano-mimetic culture plates!