SOFT CULTURE PLATES,

PLEASED CELLS.

A NEW GENERATION

OF CELL CULTURE PLATES.

At Cell&Soft, we are comitted to the research and development of soft and biomechanical substrates for in vitro cell culture. Our solutions enhance the physiology and the relevance of cell culture and cell-based assays.

Our team spent years conducting research to develop a new generation of cell culture plates that offers controlled rigidity and mechano-mimetic extracellular environments. Our mission is to reinvent cell culture, by becoming a new and innovative actor for the in vitro research.

WHY CULTURE YOUR CELLS ON SOFT SUBSTRATES?

Because our organs are one billion times stiffer than plastic.

In vitro cell-based assays are recognized as important contributors to persistent failures to translate promising drug candidates into clinical success, due, in part, to their limited predictability and reproducibility of the in vivo cell models.

In biological conditions, cells organize in tissues with distinct physical properties where they evolve on soft and textured matrices  . Matrix rigidities impact almost all aspect of cell behavior  . including transcriptional regulation  , protein synthesis  . endocytosis   and even glycolysis necessary for fast metabolism  . They play a major role in carcinogenesis and metastasis formation  , in stem cells differentiation   and in drug effectiveness  . Therefore, cell culture devices should not only allow cell survival and growth but must offer an environment as close as possible to the natural microenvironment of targeted tissues to be representative of the in vivo conditions. Up to date, cell-based assays are still performed in plastic dishes, whose rigidity is up to one billion times stiffer than the in vivo cell environment: The failures of standard culture dishes to maintain the phenotype of many primary cells, or by appearing as wrong predictors support the evidence than these standard tools have now reached their limits. ​Now let’s reinvent cell culture by using soft, mechano-mimetic culture plates!

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Mean elastic Young’s moduli of human organs.

WHY USING CELL&SOFT CULTURE PLATES?

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Cell culture

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Stem cells

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Drug discovery

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Microscopy &

time lapse

THEY TRUST US

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SOFT CULTURE PLATES,
PLEASED CELLS.

by

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Cell&Soft provides uniformly soft or rigidity textured

cell culture plates with an adhesive coating.

Discover our range of products made in France by Cell&Soft.

REFERENCES

  1. Discher DE et al. Tissue cells feel and respond to the stiffness of their substrate. Science. 2005 310:1139.

  2. Bouchonville N et al. AFM mapping of the elastic properties of brain tissue reveals kPa/μm gradients of rigidity. Soft Matter. 2016 12:6232.

  3. Mammoto A et al. Mechanosensitive mechanisms in transcriptional regulation. J Cell Sci. 2012 125:3061.

  4. Tilghman RW et al. Matrix rigidity regulates cancer cell growth by modulating cellular metabolism and protein synthesis.PLoS One. 2012 7(5):e37231.

  5. Missirlis D. The effect of substrate elasticity and actomyosin contractility on different forms of endocytosis. PLoS One. 2014 9(5):e96548.

  6. Park JS et al. Mechanical regulation of glycolysis via cytoskeleton architecture. Nature. 2020 578 :621-626

  7. Mouw JK et al. Tissue mechanics modulate microRNA-dependent PTEN expression to regulate malignant progression. Nat Med. 2014 20:360.

  8. Engler AJ et al. Matrix elasticity directs stem cell lineage specification. Cell. 2006 126(4):677-89.

  9. Zustiak SP. The Role of Matrix Compliance on Cell Responses to Drugs and Toxins: Towards Predictive Drug Screening Platforms. Macromol Biosci. 2015 15:589-99.​