HOLOGRAPHY

EXAMPLES OF APPLICATIONS

COMPATIBLE WITH HOLOGRAPHIC MICROSCOPY SYSTEMS

Courtesy of System-C Bioprocess, France and PHI AB (Phase Holography Imaging), Sweden

 

Cells: A549 (adenocarcinomic human alveolar basal epithelial cells)
Plate: Lung Mecachips™ - Collagen
Labeling: Label free
Microscopy: Holomonitor™ M4 (PHI AB (Sweden)

Distributor: System-C (France) 

https://www.system-c-bioprocess.com/produit/holomonitor-m4/
Equipment: motarized stage, low power red laser, x20 magnification objective
Time-Lapse imaging: App Suite – 1 image every 5 minutes – 24 h

SURFACE COATING OF THE POLYACRYLAMIDE HYDROGEL

Revêtement hydrogel 1.png

The human truncated recombinant vitronectin (VTN-N) is covalently grafted to the surface and does not penetrate into the hydrogel. Horizontal bar: 50µm. Vertical bar: 10µm.

Revêtement hydrogel 2.png

The surface density of the VTN-N is independent of the rigidity of the hydrogel.

HYPOPHYSIS

Hypophysis.png

Zoom on the local stiffness of a human pituitary gland (hypohysis) tissue, measured by force indentation atomic force microscopy. Adapted from 2.