EXAMPLES OF APPLICATIONS
COMPATIBLE WITH HOLOGRAPHIC MICROSCOPY SYSTEMS
Courtesy of System-C Bioprocess, France and PHI AB (Phase Holography Imaging), Sweden
Cells: A549 (adenocarcinomic human alveolar basal epithelial cells)
Plate: Lung Mecachips™ - Collagen
Labeling: Label free
Microscopy: Holomonitor™ M4 (PHI AB (Sweden)
Distributor: System-C (France)
Equipment: motarized stage, low power red laser, x20 magnification objective
Time-Lapse imaging: App Suite – 1 image every 5 minutes – 24 h
SURFACE COATING OF THE POLYACRYLAMIDE HYDROGEL
The human truncated recombinant vitronectin (VTN-N) is covalently grafted to the surface and does not penetrate into the hydrogel. Horizontal bar: 50µm. Vertical bar: 10µm.
The surface density of the VTN-N is independent of the rigidity of the hydrogel.
Zoom on the local stiffness of a human pituitary gland (hypohysis) tissue, measured by force indentation atomic force microscopy. Adapted from 2.